CLSI H 21-A5 PDF

CLSI HA5 Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays and Molecular Hemostasis Assays;. CLSI HA5. Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays; Approved Guideline. HA5 does not provide general guidelines for the performance of coagulation testing. Performance guidelines for specific coagulation assays are addressed.

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You can download and open this file to your own computer 21-5a DRM prevents opening this file on another computer, including a networked server. Need more than one copy? However, these guidelines may also be considered for other sample types e. Please review our privacy policy. The interference can be the result of various mechanisms including: If a laboratory decides to report affected tests, the H-Index levels used for these purposes must be documented.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

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We have no amendments or corrections for this standard. This standard is also available to be included in Standards Subscriptions.

Consensus Statement for the Management and Reporting of Haemolysed Specimens

Laboratories must monitor the frequency of haemolysed blood samples and investigate the source of collection to enable continuous improvement. The most frequently encountered causes of haemolysis of blood samples include use of inappropriately small gauge needles during phlebotomy and transfer of blood from a syringe into a vacutainer tube via a needle e.

Cut-offs at which assays are affected must be reviewed and updated when analytical platforms are changed. You may delete a document from your Alert Profile at any time. Clinically relevant limits can be sourced from:.

The interference can be the result of various mechanisms including:. A clear statement describing the magnitude and direction of the interference must be included. Spectral haemoglobin absorbs atand nm – leading to interference with alkaline phosphatase, gamma-glutamyl transferase, total and direct bilirubin.

This standard is not included in any packages. Support Center Support Center. Introduction Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories. Ann Acad Med Singapore. Tests of the coagulation system are very sensitive to storage time and temperatureconcentration of anticoagulant, and surface of containers; attention to these parameters is important.

This consensus statement is primarily intended as a guideline for diagnostic pathology laboratories undertaking any analyses performed on serum or plasma samples. Where multiple different analytical platforms are in use, for example across a network of laboratories or in a large central laboratory, laboratories must ensure H-Index thresholds are appropriate for each.

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When a POCT result shows a result e. Impact of Haemolysis on Test Results S3. Proceed to Checkout Continue Shopping. The H-index at which a clinically relevant effect is seen must be determined for each method in use. The interference with immunoassays is less frequent clzi with photometric assays, but is still a significant consideration; one of the most commonly affected immunoassays 21-x5 the cardiac Troponin assays. Journal List Clin Biochem Rev v.

Influence of hemolysis on routine clinical chemistry testing. Please first verify your email before subscribing to alerts. Additive – as result of greater concentrations of analytes in cells than in plasma e.

The effect of hemolysis on current troponin assays confounding preanalytical variable? Areas to consider could include data mining, guidance on how to perform in-house clso and the defining of appropriate action limits. Published clinical outcome studies describing performance requirements for the specific analyte.

Subscription pricing is determined by: Laboratories must have a means to identify affected patient results e.